Journal: BBA Advances

Article Title: Claudin-11, a hypomyelinating leukodystrophy 22 (HLD22)-responsible protein, uniquely interacts with shroom-2 to change cell phenotypes

doi: 10.1016/j.bbadva.2025.100159

Figure Lengend Snippet: Key materials used in this study.

Article Snippet: pcDNA3.1(+)-N-eGFP-human Cldn11 (last 30 mino acids) 208GlnExt39 , Genscript , J377 × 912G0–4 , RG396977 , 1.25 μg of DNA per 6 cm dish.

Techniques: Western Blot, Sequencing, Immunoprecipitation, Concentration Assay, Plasmid Preparation

Journal: BBA Advances

Article Title: Claudin-11, a hypomyelinating leukodystrophy 22 (HLD22)-responsible protein, uniquely interacts with shroom-2 to change cell phenotypes

doi: 10.1016/j.bbadva.2025.100159

Figure Lengend Snippet: Knockdown of claudin-11 decreases cell morphology and marker protein expression. (A, B) FBD-102b cells were transfected with control luciferase siRNA (siControl) or claudin-11 siRNA (siClaudin11) and cultured for 0 or 3 days following the induction of differentiation. Cells meeting differentiation criteria were counted and statistically depicted in the graph (** p < 0.01; n = 10 fields). Panels a and b (lower photographs) are enlarged images of squares a and b surrounded by black dotted lines (middle photographs). Some cells are surrounded by white dotted lines. (C and D) And, cell lysates following the induction of differentiation were immunoblotted with the respective antibodies against differentiation marker proteins MBP and PLP1, as well as the internal control actin. The immunoreactive bands of interest were quantified and graphed, with other immunoreactive bands set to 100 % (* p < 0.05; n = 3 blots).

Article Snippet: pcDNA3.1(+)-N-eGFP-human Cldn11 (last 30 mino acids) 208GlnExt39 , Genscript , J377 × 912G0–4 , RG396977 , 1.25 μg of DNA per 6 cm dish.

Techniques: Knockdown, Marker, Expressing, Transfection, Control, Luciferase, Cell Culture

Journal: BBA Advances

Article Title: Claudin-11, a hypomyelinating leukodystrophy 22 (HLD22)-responsible protein, uniquely interacts with shroom-2 to change cell phenotypes

doi: 10.1016/j.bbadva.2025.100159

Figure Lengend Snippet: Transfection of the PDZ ligand sequence of claudin-11 decreases cell morphology and marker protein expression. (A, B) Cells were transfected with a control vector (Control) or the plasmid encoding the GFP-tagged PDZ ligand sequence of claudin-11 (claudin-11 [C]) and cultured for 0 or 3 days following the induction of differentiation. Cells meeting differentiation criteria were counted and statistically depicted in the graph (*** p < 0.001; n = 10 fields). Panels a and b (lower photographs) are enlarged images of squares a and b surrounded by black dotted lines (middle photographs). Some cells are surrounded by white dotted lines. (C and D) And, cell lysates following the induction of differentiation were immunoblotted with the respective antibodies against differentiation marker proteins, tagged protein (GFP-claudin [C]), and actin. The immunoreactive bands of interest were quantified and graphed, with other immunoreactive bands set to 100 % (** p < 0.01, * p < 0.05; n = 3 blots).

Article Snippet: pcDNA3.1(+)-N-eGFP-human Cldn11 (last 30 mino acids) 208GlnExt39 , Genscript , J377 × 912G0–4 , RG396977 , 1.25 μg of DNA per 6 cm dish.

Techniques: Transfection, Sequencing, Marker, Expressing, Control, Plasmid Preparation, Cell Culture

Journal: BBA Advances

Article Title: Claudin-11, a hypomyelinating leukodystrophy 22 (HLD22)-responsible protein, uniquely interacts with shroom-2 to change cell phenotypes

doi: 10.1016/j.bbadva.2025.100159

Figure Lengend Snippet: Knockdown of claudin-11 decreases the phosphorylation level of Akt kinase. (A, B) Cells were transfected with control luciferase siRNA (siControl) or claudin-11 siRNA (siClaudin11) and allowed to differentiate for 2 days. Cell lysates were immunoblotted with the respective antibodies against phosphorylated Akt kinase (pAkt) and total Akt kinase (Akt). The immunoreactive bands of interest were quantified and graphed, with other immunoreactive bands set to 100 % (** p < 0.01; n = 3 blots).

Article Snippet: pcDNA3.1(+)-N-eGFP-human Cldn11 (last 30 mino acids) 208GlnExt39 , Genscript , J377 × 912G0–4 , RG396977 , 1.25 μg of DNA per 6 cm dish.

Techniques: Knockdown, Phospho-proteomics, Transfection, Control, Luciferase